Gram's method

Gram's method
n. Biol. a method of differentiating bacteria by staining with a dye, then attempting to remove the dye with a solvent, for purposes of identification.
Phrases and idioms:
Gram-positive (or negative) (of bacteria) that do (or do not) retain the dye.
Etymology: H. C. J. Gram, Da. physician d. 1938

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noun
a staining technique used to classify bacteria; bacteria are stained with gentian violet and then treated with Gram's solution; after being decolorized with alcohol and treated with safranine and washed in water, those that retain the gentian violet are Gram-positive and those that do not retain it are Gram-negative
• Hypernyms: ↑staining

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\\ˈgramz-, -aa(ə)mz-\ noun or gram method
Usage: capitalized G
Etymology: after Hans C. J. Gram
: a method for the differential staining of bacteria by which they are treated with Gram's solution after being stained with gentian violet and are then treated with alcohol and washed in water with the result that certain species retain the dye while others are decolorized — see gram-negative, gram-positive

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/gramz/, (sometimes l.c.)
a method of staining and distinguishing bacteria, in which a fixed bacterial smear is stained with crystal violet, treated with Gram's solution, decolorized with alcohol, counterstained with safranine, and washed with water. Cf. Gram-negative, Gram-positive.
[named after Hans C. J. Gram (1853-1938), Danish bacteriologist]

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Gram's method «gramz»,
a method of classifying bacteria by first staining them with gentian violet, treating them with Gram's solution, and washing them with alcohol. Gram-positive bacteria retain the violet dye; Gram-negative species lose it.
╂[< Hans C. J. Gram, 1853-1938, a Danish bacteriologist, who discovered it]

Useful english dictionary. 2012.

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Look at other dictionaries:

  • Gram's method — [gramz] n. [after Hans C. J. Gram (1853 1938), Dan physician] a method of staining bacteria for the purpose of classification, involving treatment with crystal violet, an iodine solution, and alcohol: the violet stain is retained by Gram positive …   English World dictionary

  • Gram's method — Gram s′ meth′od [[t]græmz[/t]] n. lab (sometimes l.c.) a method of characterizing bacteria that involves staining a slide of fixed specimens with gentian violet, washing with alcohol, and applying a counterstain • Etymology: after Hans C. J. Gram …   From formal English to slang

  • Gram's method — /gramz/, (sometimes l.c.) a method of staining and distinguishing bacteria, in which a fixed bacterial smear is stained with crystal violet, treated with Gram s solution, decolorized with alcohol, counterstained with safranine, and washed with… …   Universalium

  • Gram's method — /ˈgræmz mɛθəd/ (say gramz methuhd) noun a method of bacterial staining in which the film is first stained with crystal violet and then with Gram s iodine solution. It permits the classification of bacteria, Gram positive species keeping the… …  

  • Gram's method — noun a staining technique, using a sequence of crystal violet, iodine and safranine, used to differentiate bacteria into two large groups of species Syn: Gram staining …   Wiktionary

  • Gram's method — noun see Gram s stain …   New Collegiate Dictionary

  • Gram staining — (or Gram s method) is an empirical method of differentiating bacterial species into two large groups (Gram positive and Gram negative) based on the chemical and physical properties of their cell walls.cite book | last = Bergey | first = David H.… …   Wikipedia

  • Gram-negative — bacteria lose the crystal violet stain (and take the color of the red counterstain) in Gram s method of staining. This is characteristic of bacteria that have a cell wall composed of a thin layer of a particular substance (called peptidoglycan).… …   Medical dictionary

  • Gram-positive — bacteria retain the color of the crystal violet stain in the Gram stain. This is characteristic of bacteria that have a cell wall composed of a thick layer of a particular substance (called peptidologlycan). The Gram positive bacteria include… …   Medical dictionary

  • Gram's stain — or Gram stain gram n 1) a method for the differential staining of bacteria that involves fixing the bacterial cells to a slide and staining usu. with the basic dye crystal violet, treating with an iodine solution to fix the dye, washing with… …   Medical dictionary

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